There are few historical sources relating to this nature reserve, the reason perhaps being the distance of the site from major settlements in the area. It is possible that the prophet Ezekiel is referring these springs in one of his prophecies about the Dead Sea becoming a fishing site, calling them 'En Eglayim' – "Fishermen will stand along the shore, from En Gedi to En Eglayim" (Ezekiel 47:10).

Just a few kilometers from Enot Tsukim is Qumran National Park, which became famous after the discovery of the Dead Sea Scrolls.

During the 19th century, European researchers came to the Dead Sea region. One of the most notable of these was the Scottish priest Henry Baker Tristram, who was called the "father of fauna and flora in the Holy Land", and who described the place with great enthusiasm. In his book "In the Land of Israel – a journal of travels 1863 – 1864", Tristram wrote that he stopped in the area with his scientific delegation and for two days, delighted in the sights.